Efficient Procedure for Induction Somatic Embryogenesis in Holm Oak: Roles of Explant Type, Auxin Type, and Exposure Duration to Auxin

نویسندگان

چکیده

Holm oak is the dominant tree species in Mediterranean climate. Currently, worrisome degradation of its ecosystems has been observed, produced, among other factors, by changes land use, extreme weather events, forest fires, climate change, and especially increasingly frequent episodes high mortality caused “oak decline”, which brought with it a social concern that transcends productive interest. Breeding conservation programs for this are necessary to ensure prevalence these future generations. Biotechnological tools such as somatic embryogenesis (SE) have great potential value improvement shown be highly efficient propagation woody species. One challenge approach SE induction holm not yet optimized. Here, we present new reproducible procedure induce oak; evaluated responsiveness different initial explants exposed types, concentrations, durations auxin. rates were significantly improved (37%) culturing nodal segments two weeks medium. In addition, significant auxin–genotype interaction was observed.

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ژورنال

عنوان ژورنال: Forests

سال: 2023

ISSN: ['1999-4907']

DOI: https://doi.org/10.3390/f14020430